EVALUATION OF FUNGAL ISOLATES FROM ORAL SAMPLES IN PATIENTS WITH THALASSEMIA

Abstract

This research was carried out for biochemical diagnosis of fungal species taken from Thalassemic subjects in AL-Najaf City and assessment of some antifungal agents (Amphotericin B and Nystatin). From January through December 2023, fifty samples were obtained from Al-Sadr Medical City in Al_Najaf City, and only fourteen samples showed positive growth. All isolates were cultivated in on chromagar medium after culture on Sabouraud dextrose agar for identification of different species of Candida by color of the colonies. We can consider they yeast species as the most common fungal etiological agent of life threatening invasive infections in Thalassemia patients who are severely immunocompromised and treatment which requires extended stay in intensive care units Results in this study confirmed the presence of 10 colonies of C. tropicalis1(7.1%), C. parapsilosis 3(21.4%), and C. albicans 10(71.4%), and finally. In this study, C. albicans was investigated for the production of some pathogenesis factors such as biofilms and germ tubes and enzymes-phospholipase during culture of Candida spp. on several cultures. Cultivation of all samples  on Sabouraud dextrose agar SDA and then on CHROMagar medium for the purpose of diagnosing the species of Candida depending on the color of the colonies. Findings of fungal growth exhibit that C. albicans forms all pathogenic factors including phospholipase enzyme, germ tube, and biofilms. The finding of this research concerning antifungal activity for (Nystatin and Amphotercin B), shows the impacts on Candida spp. by utilizing the disk diffusion method. Findings  also exhibit that Amphotercin B exhibited more antifungal activity than Nystatin against Candida spp. he results were diagnosed on CHROMagar medium as follows: C.albicans 10(71.4%), followed by C. parapsilosis 3(21.4%), C. tropicalis1(7.1%).In this study, the ability of C.albicans was tested to produce some virulence factors such as germ tube, biofilms and production of enzymes phospholipases when culturing Candida spp. on different media.