Evaluation The MIR26 As a Bio Marker in Rheumatoid Arthritis Patients

Abstract

Background: Rheumatoid arthritis, also known as RA, is a systemic inflammatory autoimmune disease that is persistent and affects 1% of people globally. Aim of study: This study was conducted to calculation the expression of miRNA-26 by RT-PCR. Methodology: A case control study has been conducted from 15 October 2023 to 15 of January 2024. This study has been done at the Medical City Hospital, Baghdad Government. And included 45 patients ( females and male)  which diagnosed with RA disease by specialist physician, and their ages ranges between, 18 to 75 years. Blood was drawn from the patients to investigate the MIR26, by RT- PCR (Real time polymerase chain reaction). Results: The average Ct value for miR-26 in the group of controls is 6.82, with a fold change of around 1.00 while the average Ct value for miR-26 in group of patients is 7.59, with a fold change of around 7.59. Conclusion: There is significance elevation in miRNA26 in patients group other than in control subjects' group. It is possible that miR-26 contributes to the diseases or disorders that these people suffer from it.