Identification of Methicillin Resistant Staphylococcus Aureus Isolated from Chronic Suppurative Otitis Media

Abstract

Introduction: Staphylococcus aureus  isolated from prolonged suppurative otitis media was the focus on the current investigation. Aims: The goal of this study is the identification of methicillin resistant Staphylococcus aureus isolated from chronic suppurative otitis media. Material and methods: The research population is made up of 100 medical samples were taken from individual with chronic otitis media infection who visited Alfurat Al-Awsat Hospital and AL-Zahra Teaching Hospital for Children in the province of Al-Najaf between January 1, 2023, and March 20, 2023. Results: The samples were injected onto mannitol salt agar (MSA) and then subcultured on blood agar after being incubated for (24 to 48 hours at 37°C). Characterization of colonies, Gram's staining, and conventional biochemical testing. A genomic investigation of MRSA isolates was accompanied using PCR affecting the mecA gene. Staphylococcus aureus bacteria were create in 34.5% of specimens. Genotyping complete polymerase chain reaction and biochemical examination presented 58% correctness in recognizing detaches. 20 bacterial specimens were found to encompass the mecA gene, representative a joining among chronic suppurative otitis media and MRSA resounding the mecA gene. Conclusion: this study show Staphylococcus aureus is more causative agent for  suppurative otitis media and resistant for some antibiotic because resistant to  mecA gene.