Evaluation of Some Immunological Parameters Associated with Diabetic Foot Infection in Kerbala Governorate

Abstract

Diabetes mellitus is a chronic disorder that lasts to amplify considerably. One of its numerous complications is Diabetic foot infection (DFI) that may cause several alterations in immunological parameters’ value. This study aimed to evaluate some immunological parameters including Toll like Receptor-2, Interleukin-17A and C-reactive protein in patients of type two diabetes mellitus with or without diabetic foot ulcers and in healthy individuals in Kerbala city. The current study, performed at Imam Al-Hassan Center for Endocrinology and Diabetes in Karbala, Iraq, for 120 patients of diabetic foot of both sexes and diverse ages starts from 35 years- ≥ 75.  Seven ml of blood have drained from participants though a disposable syringe. The blood has been placed inside a gel tubes and centrifuged at 4000 xg in cooling centrifuge to get serum. The serum was put into an Eppendorf tubes and kept at (-20˚C) until using it. The mean level of Toll-like receptor 2 (TLR-2) in DFI patients was (7.36±1.85) ng/ml which was significantly higher than in  DM patients and control groups (p ≤ 0.001) while in interleukin-17A (IL-17A), the mean of levels in DFI patients were (123.7±33.52) ng/L which was significantly higher than DM and control groups (P ≤ 0.001) and C-reactive protein (CRP) showed a higher rate in DFI patients (92.9±78.26) mg/L which was significantly higher than DM and control groups (p ≤ 0.001). In Conclusions: Our current study displays that the Presence of significant elevated levels of immunological markers like TLR-2,  IL-17A and CRP in DFI patients in comparison to DM and Control groups could support a positive role of these markers in T2DM pathogenesis.