Determination of Aflatoxin in the Blood of Patients with Hepatopathy and Evaluation its Effect on some Immune Parameters
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Abstract
Background: Aflatoxin-producing Aspergillus species, Aspergillus flavus and Aspergillus parasiticus, are a main producer of aflatoxins, which a class of toxic molds that can cause cancer and other health issues. Aims of study: Serum aflatoxin B1 detection, both qualitative and quantitative by using TLC and HPLC techniques; serum cytokine levels are used to assess the aflatoxin's impact on the human immune system. Methods: A study was carried out between November 2023 and January 2024. Blood samples were drawn from 150 Volunteer patients who were receiving care at Tikrit hospitals. Liver function tests (AST and ALT) were measure in the hospital used an automated device, ELISA test was used to detect IL-4, TNF-a, and IL-10, while detection of serum aflatoxin B1 was done both qualitatively and quantitatively using TLC and HPLC procedures. Results and Discussion; Out of 150 sample, the results showed 87 (58%) patients with (3.995±0.450) level of Aflatoxin B1 compared with 63(42%) patients of non-AFB1 group which scored (0.14±0.007) level of AFB1, these analyses were performed using HPLC. The study results showed increasing serum liver enzymes of AFB1 group compared with non AFB1 group, the pro-inflammatory cytokines increased (16.01±1.31) for AFB1, while anti-inflammatory cytokines (IL-4, IL-10) decreased (9.96*±1.54, 4.90*±1.23) respectively. Conclusion: The study's findings suggest that toxins build up over time and can interfere with a variety of biological processes, any amount of toxin in the body is harmful. Aflatoxin changes pro- and anti-inflammatory cytokines and affects liver function.
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