https://bcsdjournals.com/index.php/jsrmbs/issue/feed 2025-07-30T14:14:18+07:00 Prof. Feng-Huei Lin editor@bcsdjournals.com Open Journal Systems <p><strong>Journal of Scientific Research in Medical and Biological Sciences ISSN 2709-0159(print) and ISSN 2709-1511 (online)</strong> is an open access international journal dedicated to the latest advancement of medical and biochemical sciences. It publishes high-quality theoretical and applied research ranging from scientific research to application development. <strong>JSRMBS</strong> highlights the efficient and effective medical science and biomedical applications and provides a central forum for a deeper understanding in the discipline by encouraging the quantitative comparison and performance evaluation of the emerging components of these domains.</p> <p><strong>JSRMBS </strong>also emphasizes all prospective &amp; applications of medical and biological sciences and promulgates both the original research papers and industrial experience-analysis studies and reports with a view to provide scholarly research in original research papers and real-time innovations, development, and advancement in different disciplines of medical sciences and biomedical innovations.</p> <ul> <li><strong>P-ISSN</strong>: <strong>2709-0159</strong></li> <li><strong>E-ISSN</strong>: <strong>2709-1511</strong></li> <li><strong>DOI:</strong> Yes (10.47631)</li> <li><strong>Frequency: </strong>Quarterly</li> <li><strong>Publication Dates:</strong> | February, May, August, November|</li> <li><strong>Format</strong>:<strong> </strong>Online &amp; Print</li> <li><strong>Scope: </strong>General Medicine, Public Health, Biomedical Sciences, Biological Sciences</li> <li><strong>Open Access: </strong>Yes</li> <li><strong>Indexed: </strong>Yes</li> <li><strong>Policy: </strong>Peer-Reviewed/Refereed</li> <li><strong>Review</strong> <strong>Time: </strong>Four Weeks Approximately</li> <li><strong>E-mail: </strong><a href="mailto:editor@bcsdjournals.com">editor@bcsdjournals.com</a></li> </ul> https://bcsdjournals.com/index.php/jsrmbs/article/view/959 2025-07-23T15:55:27+07:00 Jawad N. K. Makassees jawadnkm25@yahoo.com

<p>&nbsp;&nbsp; In this study, the author is concerned with the purification, extraction and biochemical analysis of arginine deiminase (ADI) of a clinical strain of <em>Escherichia coli</em> to determine its anticancer properties. Ammonium sulfate precipitation followed by two subsequent purification processes such as Ion-exchange chromatography and gel filtration enhanced the purity 9.5 fold and the final specific activity was found to be 16 U/ mg with the molecular weight of 46 kDa . The enzyme was observed to be most active at pH 7.0 and 37^oC and lost less than 75% of its trial activities at marginally acidic to neutral pH. Using metal ion analysis and the fact that ADI was a metalloenzyme; Fe ³⁺ and Mn ²⁺ were found to increase the ADI activity, whereas EDTA suppressed the activity significantly. In vitro cytotoxic testing by MTT technique showed that ADI dose dependently and specifically suppressed the viability of HeLa cancer cells with the IC₅₀ of 140.55 ug/mL, whereas the normal HEK293 cells had the IC₅₀ of 253.47 ug/mL, with a selectivity index of 1.80. This justifies the therapeutic potential of bacterial ADI as a specific anticancer agent of arginine-auxotrophic tumors.</p>

2025-07-30T00:00:00+07:00 Copyright (c) 2025 Jawad N. K. Makassees https://bcsdjournals.com/index.php/jsrmbs/article/view/961 2025-07-23T18:40:30+07:00 Najlaa Kadhim Ali njlaakd@gmail.com

<p>Chronic kidney disease represents the predominant etiology of chronic metabolic acidosis. Reduced tubular ammoniogenesis, or the loss of nephron mass, is the main cause of the decrease in the tubular net secretion of protons. Numerous problems related to cMA have been explored recently. These included the breakdown of proteins, decreased production of new proteins, demineralization of bone, inflammation, and the advancement of chronic kidney disease. Foods like cheese, meat, eggs, and grains often increase the amount of acid in the diet, whereas fruits and vegetables (F+V) are thought to produce bases. <strong>Conclusions:</strong> The most common cause of chronic metabolic acidosis is chronic renal illness. Chronic metabolic acidosis linked to CKD most likely worsens the course of chronic kidney disease. Rather than metabolic acidosis, insufficient hemodialysis is the cause of malnutrition in chronic hemodialysis patients.&nbsp; Patients with kidney disorders can effectively manage metabolic acidosis by increasing their fruit and vegetable consumption and taking oral alkali supplements.</p>

2025-07-30T00:00:00+07:00 Copyright (c) 2025 Najlaa Kadhim Ali